Analisis Hambatan Siklus Sel Kanker Lidah Manusia SP-C1 yang Diinduksi oleh Biskoklaurin Alkaloid Cepharantine Menggunakan flow cytometry
Abstract
Flow cytometry has been widely used for detecting of apoptosis and cell cycle arrest on several human cancer cells. Supris clone-1 (SP-C1) cell is an oral tongue squamous cell carcinoma cell line which has rapidly growth, highly migration-invasion and metastatic to regional and distant lymph nodes. The aim of the study was to analyze the cell cycle arrest of SP-C1 inducted by cepharantine (CE) using flow cytometry. Treatment of SP-C1 cells with CE concentration 0, 5, 10 and 20 g/ml was carried out for 72 hours. The examination of cell cycle arrest of SP-C1 by flow cytometry revealed that CE with concentration 10 and 20 g/ml was significantly arrested of SP-C1 cell cycle in G1-S phase. Moreover, apoptosis was detected in G0 phase on the cells treated by CE 20 g/ml. These results concluded that CE had a potency to increase apoptosis with suppressing SP-C1 cell cycle phases.
DOI: 10.33371/ijoc.v4i3.103
Article Metrics
Abstract View: 350,Refbacks
- There are currently no refbacks.
Copyright (c) 2014 Indonesian Journal of Cancer